Carter, P. J. & Lazar, G. A. Next generation antibody drugs: pursuit of the ‘high-hanging fruit’. Nat. Rev. Drug Discov. 17, 197–223 (2018).
Google Scholar
Verdine, G. L. & Walensky, L. D. The challenge of drugging undruggable targets in cancer: lessons learned from targeting BCL-2 family members. Clin. Cancer Res. 13, 7264–7270 (2007).
Google Scholar
Stewart, M. P. et al. In vitro and ex vivo strategies for intracellular delivery. Nature 538, 183–192 (2016).
Google Scholar
Samal, S. K. et al. Cationic polymers and their therapeutic potential. Chem. Soc. Rev. 41, 7147–7194 (2012).
Google Scholar
Brooks, H., Lebleu, B. & Vivès, E. Tat peptide-mediated cellular delivery: back to basics. Adv. Drug Del. Rev. 57, 559–577 (2005).
Google Scholar
Peraro, L. & Kritzer, J. A. Emerging methods and design principles for cell-penetrant peptides. Angew. Chem. Int. Ed. 57, 11868–11881 (2018).
Google Scholar
Pei, D. & Buyanova, M. Overcoming endosomal entrapment in drug delivery. Bioconjug. Chem. 30, 273–283 (2019).
Google Scholar
Fu, A., Tang, R., Hardie, J., Farkas, M. E. & Rotello, V. M. Promises and pitfalls of intracellular delivery of proteins. Bioconjug. Chem. 25, 1602–1608 (2014).
Google Scholar
Fawell, S. et al. Tat-mediated delivery of heterologous proteins into cells. Proc. Natl Acad. Sci. USA 91, 664–668 (1994).
Google Scholar
Cornelissen, B. et al. Imaging DNA damage in vivo using γH2AX-targeted immunoconjugates. Cancer Res. 71, 4539–4549 (2011).
Google Scholar
Singh, K., Ejaz, W., Dutta, K. & Thayumanavan, S. Antibody delivery for intracellular targets: emergent therapeutic potential. Bioconjug. Chem 30, 1028–1041 (2019).
Google Scholar
Brock, R. The uptake of arginine-rich cell-penetrating peptides: putting the puzzle together. Bioconj. Chem. 25, 863–868 (2014).
Google Scholar
Madani, F., Lindberg, S., Langel, U., Futaki, S. & Gräslund, A. Mechanisms of cellular uptake of cell-penetrating peptides. J. Biophys. 2011, 414729 (2011).
Google Scholar
Dougherty, P. G., Sahni, A. & Pei, D. Understanding cell penetration of cyclic peptides. Chem. Rev. 119, 10241–10287 (2019).
Google Scholar
Milletti, F. Cell-penetrating peptides: classes, origin and current landscape. Drug Discov. Today 17, 850–860 (2012).
Google Scholar
Nischan, N. et al. Covalent attachment of cyclic TAT peptides to GFP results in protein delivery into live cells with immediate bioavailability. Angew. Chem. Int. Ed. 54, 1950–1953 (2015).
Google Scholar
Herce, H. D. et al. Cell-permeable nanobodies for targeted immunolabelling and antigen manipulation in living cells. Nat. Chem. 9, 762–771 (2017).
Google Scholar
Schneider, A. F. L., Kithil, M., Cardoso, M. C., Lehmann, M. & Hackenberger, C. P. R. Cellular uptake of large biomolecules enabled by cell-surface-reactive cell-penetrating peptide additives. Nat. Chem. 13, 530–539 (2021).
Google Scholar
Akishiba, M. et al. Cytosolic antibody delivery by lipid-sensitive endosomolytic peptide. Nat. Chem. 9, 751–761 (2017).
Google Scholar
Ovacik, M. & Lin, K. Tutorial on monoclonal antibody pharmacokinetics and its considerations in early development. Clin. Transl. Sci. 11, 540–552 (2018).
Google Scholar
Kauffman, W. B., Fuselier, T., He, J. & Wimley, W. C. Mechanism matters: a taxonomy of cell penetrating peptides. Trends Biochem. Sci. 40, 749–764 (2015).
Google Scholar
Herce, H. D. & Garcia, A. E. Molecular dynamics simulations suggest a mechanism for translocation of the HIV-1 TAT peptide across lipid membranes. Proc. Natl Acad. Sci. USA 104, 20805–20810 (2007).
Google Scholar
Lawrence, M. S., Phillips, K. J. & Liu, D. R. Supercharging proteins can impart unusual resilience. J. Am. Chem. Soc. 129, 10110–10112 (2007).
Google Scholar
Cronican, J. J. et al. Potent delivery of functional proteins into mammalian cells in vitro and in vivo using a supercharged protein. ACS Chem. Biol. 5, 747–752 (2010).
Google Scholar
Freire, J. M., Almeida Dias, S., Flores, L., Veiga, A. S. & Castanho, M. A. R. B. Mining viral proteins for antimicrobial and cell-penetrating drug delivery peptides. Bioinformatics 31, 2252–2256 (2015).
Google Scholar
Tung, C.-H., Mueller, S. & Weissleder, R. Novel branching membrane translocational peptide as gene delivery vector. Biorg. Med. Chem. 10, 3609–3614 (2002).
Google Scholar
Angeles-Boza, A. M., Erazo-Oliveras, A., Lee, Y.-J. & Pellois, J.-P. Generation of endosomolytic reagents by branching of cell-penetrating peptides: tools for the delivery of bioactive compounds to live cells in cis or trans. Bioconjug. Chem. 21, 2164–2167 (2010).
Google Scholar
Fu, J., Yu, C., Li, L. & Yao, S. Q. Intracellular delivery of functional proteins and native drugs by cell-penetrating poly(disulfide)s. J. Am. Chem. Soc. 137, 12153–12160 (2015).
Google Scholar
Erazo-Oliveras, A. et al. Protein delivery into live cells by incubation with an endosomolytic agent. Nat. Methods 11, 861–867 (2014).
Google Scholar
Erazo-Oliveras, A. et al. The late endosome and its lipid BMP act as gateways for efficient cytosolic access of the delivery agent dfTAT and its macromolecular cargos. Cell Chem. Biol. 23, 598–607 (2016).
Google Scholar
Najjar, K. et al. Unlocking endosomal entrapment with supercharged arginine-rich peptides. Bioconj. Chem. 28, 2932–2941 (2017).
Google Scholar
Kez, C., Lin, H., Peter, D. W. & Arwyn, T. J. Endocytosis, intracellular traffic and fate of cell penetrating peptide based conjugates and nanoparticles. Curr. Pharm. Des. 19, 2878–2894 (2013).
Jonkman, J., Brown, C. M., Wright, G. D., Anderson, K. I. & North, A. J. Tutorial: guidance for quantitative confocal microscopy. Nat. Protoc. 15, 1585–1611 (2020).
Google Scholar
Herbert, A. GDSC colocalisation plugins http://www.sussex.ac.uk/gdsc/intranet/microscopy/UserSupport/AnalysisProtocol/imagej/colocalisation (2013).
Costes, S. V. et al. Automatic and quantitative measurement of protein-protein colocalization in live cells. Biophys. J. 86, 3993–4003 (2004).
Google Scholar
Ramirez, O., García, A., Rojas, R., Couve, A. & Härtel, S. Confined displacement algorithm determines true and random colocalization in fluorescence microscopy. J. Microsc. 239, 173–183 (2010).
Google Scholar
Söderberg, O. et al. Direct observation of individual endogenous protein complexes in situ by proximity ligation. Nat. Methods 3, 995–1000 (2006).
Google Scholar
Rouet, R. et al. Receptor-mediated delivery of CRISPR-Cas9 endonuclease for cell-type-specific gene editing. J. Am. Chem. Soc. 140, 6596–6603 (2018).
Google Scholar
Qian, Z. et al. Discovery and mechanism of highly efficient cyclic cell-penetrating peptides. Biochemistry 55, 2601–2612 (2016).
Google Scholar
Liu, H., Gaza-Bulseco, G., Faldu, D., Chumsae, C. & Sun, J. Heterogeneity of monoclonal antibodies. J. Pharm. Sci. 97, 2426–2447 (2008).
Google Scholar
Delavoie, F., Soldan, V., Rinaldi, D., Dauxois, J. Y. & Gleizes, P. E. The path of pre-ribosomes through the nuclear pore complex revealed by electron tomography. Nat. Commun. 10, 497 (2019).
Google Scholar
Paci, G., Zheng, T., Caria, J., Zilman, A. & Lemke, E. A. Molecular determinants of large cargo transport into the nucleus. eLife 9, e55963 (2020).
Google Scholar
Avrameas, A., Ternynck, T., Nato, F., Buttin, G. & Avrameas, S. Polyreactive anti-DNA monoclonal antibodies and a derived peptide as vectors for the intracytoplasmic and intranuclear translocation of macromolecules. Proc. Natl Acad. Sci. USA 95, 5601–5606 (1998).
Google Scholar
Gordon, R. E., Nemeth, J. F., Singh, S., Lingham, R. B. & Grewal, I. S. Harnessing SLE autoantibodies for intracellular delivery of biologic therapeutics. Trends Biotechnol. 39, 298–310 (2021).
Google Scholar
Bernardes, N. E. & Chook, Y. M. Nuclear import of histones. Biochem. Soc. Trans. 48, 2753–2767 (2020).
Google Scholar
Placek, B. J., Harrison, L. N., Villers, B. M. & Gloss, L. M. The H2A.Z/H2B dimer is unstable compared to the dimer containing the major H2A isoform. Protein Sci. 14, 514–522 (2005).
Google Scholar
