Kempf, H. & Zweigerdt, R. Scalable cardiac differentiation of pluripotent stem cells using specific growth factors and small molecules. Adv. Biochem. Eng. Biotechnol. 163, 39–69 (2018).
Google Scholar
Knorr, D. A. et al. Clinical-scale derivation of natural killer cells from human pluripotent stem cells for cancer therapy. Stem Cells Transl. Med. 2, 274–283 (2013).
Google Scholar
Ito, Y. et al. Turbulence activates platelet biogenesis to enable clinical scale ex vivo production. Cell 174, 636–648.e618 (2018).
Google Scholar
Wynn, T. A., Chawla, A. & Pollard, J. W. Macrophage biology in development, homeostasis and disease. Nature 496, 445–455 (2013).
Google Scholar
Hetzel, M., Ackermann, M. & Lachmann, N. Beyond “big eaters”: the versatile role of alveolar macrophages in health and disease. Int. J. Mol. Sci. https://doi.org/10.3390/ijms22073308 (2021).
Singanayagam, A. & Triantafyllou, E. Macrophages in chronic liver failure: diversity, plasticity and therapeutic targeting. Front. Immunol. 12, 661182 (2021).
Google Scholar
Ginhoux, F. & Guilliams, M. Tissue-resident macrophage ontogeny and homeostasis. Immunity 44, 439–449 (2016).
Google Scholar
Haake, K., Ackermann, M. & Lachmann, N. Concise review: towards the clinical translation of induced pluripotent stem cell-derived blood cells-ready for take-off. Stem Cells Transl. Med. 8, 332–339 (2019).
Google Scholar
Lee, C. Z. W., Kozaki, T. & Ginhoux, F. Studying tissue macrophages in vitro: are iPSC-derived cells the answer? Nat. Rev. Immunol. 18, 716–725 (2018).
Google Scholar
Ackermann, M., Dragon, A. C. & Lachmann, N. The immune-modulatory properties of iPSC-derived antigen-presenting cells. Transfus. Med. Hemother. 47, 444–453 (2020).
Google Scholar
Happle, C. et al. Pulmonary transplantation of human induced pluripotent stem cell-derived macrophages ameliorates pulmonary alveolar proteinosis. Am. J. Respir. Crit. Care Med. 198, 350–360 (2018).
Google Scholar
Xu, R. et al. Human iPSC-derived mature microglia retain their identity and functionally integrate in the chimeric mouse brain. Nat. Commun. 11, 1577 (2020).
Google Scholar
Ackermann, M. et al. Bioreactor-based mass production of human iPSC-derived macrophages enables immunotherapies against bacterial airway infections. Nat. Commun. 9, 5088 (2018).
Google Scholar
Karlsson, K. R. et al. Homogeneous monocytes and macrophages from human embryonic stem cells following coculture-free differentiation in M-CSF and IL-3. Exp. Hematol. 36, 1167–1175 (2008).
Google Scholar
van Wilgenburg, B., Browne, C., Vowles, J. & Cowley, S. A. Efficient, long term production of monocyte-derived macrophages from human pluripotent stem cells under partly-defined and fully-defined conditions. PLoS One 8, e71098 (2013).
Google Scholar
Lachmann, N. et al. Large-scale hematopoietic differentiation of human induced pluripotent stem cells provides granulocytes or macrophages for cell replacement therapies. Stem Cell Rep. 4, 282–296 (2015).
Google Scholar
Ackermann, M. et al. A 3D iPSC-differentiation model identifies interleukin-3 as a regulator of early human hematopoietic specification. Haematologica 106, 1354–1367 (2021).
Google Scholar
Dreyer, A. K. et al. TALEN-mediated functional correction of X-linked chronic granulomatous disease in patient-derived induced pluripotent stem cells. Biomaterials 69, 191–200 (2015).
Google Scholar
Lachmann, N. et al. Gene correction of human induced pluripotent stem cells repairs the cellular phenotype in pulmonary alveolar proteinosis. Am. J. Respir. Crit. Care Med. 189, 167–182 (2014).
Google Scholar
Haake, K. et al. Patient iPSC-derived macrophages to study inborn errors of the IFN-γ responsive pathway. Cells https://doi.org/10.3390/cells9020483 (2020).
Neehus, A. L. et al. Impaired IFNγ-signaling and mycobacterial clearance in IFNγR1-deficient human iPSC-derived macrophages. Stem Cell Rep. 10, 7–16 (2018).
Google Scholar
Dannenmann, B. et al. iPSC modeling of stage-specific leukemogenesis reveals BAALC as a key oncogene in severe congenital neutropenia. Cell Stem Cell https://doi.org/10.1016/j.stem.2021.03.023 (2021).
Dannenmann, B. et al. Human iPSC-based model of severe congenital neutropenia reveals elevated UPR and DNA damage in CD34+ cells preceding leukemic transformation. Exp. Hematol. 71, 51–60 (2019).
Google Scholar
Le Voyer, T. et al. Inherited deficiency of stress granule ZNFX1 in patients with monocytosis and mycobacterial disease. Proc. Natl Acad. Sci. USA https://doi.org/10.1073/pnas.2102804118 (2021).
Makaryan, V. et al. Elastase inhibitors as potential therapies for ELANE-associated neutropenia. J. Leukoc. Biol. 102, 1143–1151 (2017).
Google Scholar
Pittermann, E. et al. Gene correction of HAX1 reversed Kostmann disease phenotype in patient-specific induced pluripotent stem cells. Blood Adv. 1, 903–914 (2017).
Google Scholar
Kropp, C. et al. Impact of feeding strategies on the scalable expansion of human pluripotent stem cells in single-use stirred tank bioreactors. Stem Cells Transl. Med. 5, 1289–1301 (2016).
Google Scholar
Manstein, F. et al. High density bioprocessing of human pluripotent stem cells by metabolic control and in silico modeling. Stem Cells Transl. Med. https://doi.org/10.1002/sctm.20-0453 (2021).
Halloin, C. et al. Continuous WNT control enables advanced hPSC cardiac processing and prognostic surface marker identification in chemically defined suspension culture. Stem Cell Rep. https://doi.org/10.1016/j.stemcr.2019.09.001 (2019).
Kempf, H., Kropp, C., Olmer, R., Martin, U. & Zweigerdt, R. Cardiac differentiation of human pluripotent stem cells in scalable suspension culture. Nat. Protoc. 10, 1345–1361 (2015).
Google Scholar
Olmer, R. et al. Differentiation of human pluripotent stem cells into functional endothelial cells in scalable suspension culture. Stem Cell Rep. 10, 1657–1672 (2018).
Google Scholar
Sahabian, A., Dahlmann, J., Martin, U. & Olmer, R. Production and cryopreservation of definitive endoderm from human pluripotent stem cells under defined and scalable culture conditions. Nat. Protoc. 16, 1581–1599 (2021).
Google Scholar
Buchrieser, J., James, W. & Moore, M. D. Human induced pluripotent stem cell-derived macrophages share ontogeny with MYB-independent tissue-resident macrophages. Stem Cell Rep. 8, 334–345 (2017).
Google Scholar
Rafiei Hashtchin, A. et al. Human iPSC-derived macrophages for efficient Staphylococcus aureus clearance in a murine pulmonary infection model. Blood Adv. https://doi.org/10.1182/bloodadvances.2021004853 (2021).
Fattorelli, N. et al. Stem-cell-derived human microglia transplanted into mouse brain to study human disease. Nat. Protoc. 16, 1013–1033 (2021).
Google Scholar
Capotondo, A. et al. Intracerebroventricular delivery of hematopoietic progenitors results in rapid and robust engraftment of microglia-like cells. Sci. Adv. 3, e1701211 (2017).
Google Scholar
Bird, T. G. et al. Bone marrow injection stimulates hepatic ductular reactions in the absence of injury via macrophage-mediated TWEAK signaling. Proc. Natl Acad. Sci. USA 110, 6542–6547 (2013).
Google Scholar
Moroni, F. et al. Safety profile of autologous macrophage therapy for liver cirrhosis. Nat. Med. 25, 1560–1565 (2019).
Google Scholar
Klichinsky, M. et al. Human chimeric antigen receptor macrophages for cancer immunotherapy. Nat. Biotechnol. 38, 947–953 (2020).
Google Scholar
Ackermann, M. et al. Restored macrophage function ameliorates disease pathophysiology in a mouse model for IL10 receptor deficient very early onset inflammatory bowel disease. J. Crohns Colitis https://doi.org/10.1093/ecco-jcc/jjab031 (2021).
Ackermann, M. et al. Ex vivo generation of genetically modified macrophages from human induced pluripotent stem cells. Transfus. Med. Hemother. 44, 135–142 (2017).
Google Scholar
Lopez-Yrigoyen, M. et al. Genetic programming of macrophages generates an in vitro model for the human erythroid island niche. Nat. Commun. 10, 881 (2019).
Google Scholar
Ackermann, M. et al. A 3D iPSC-differentiation model identifies interleukin-3 as a regulator of early human hematopoietic specification. Haematologica https://doi.org/10.3324/haematol.2019.228064 (2020).
Bernecker, C. et al. Enhanced ex vivo generation of erythroid cells from human induced pluripotent stem cells in a simplified cell culture system with low cytokine support. Stem Cells Dev. 28, 1540–1551 (2019).
Google Scholar
Choi, K. D., Vodyanik, M. & Slukvin, I. I. Hematopoietic differentiation and production of mature myeloid cells from human pluripotent stem cells. Nat. Protoc. 6, 296–313 (2011).
Google Scholar
Doulatov, S. et al. Induction of multipotential hematopoietic progenitors from human pluripotent stem cells via respecification of lineage-restricted precursors. Cell Stem Cell 13, 459–470 (2013).
Google Scholar
Vo, L. T. et al. Regulation of embryonic haematopoietic multipotency by EZH1. Nature 553, 506–510 (2018).
Google Scholar
Gutbier, S. et al. Large-scale production of human iPSC-derived macrophages for drug screening. Int. J. Mol. Sci. https://doi.org/10.3390/ijms21134808 (2020).
Lancaster, M. A. & Knoblich, J. A. Organogenesis in a dish: modeling development and disease using organoid technologies. Science 345, 1247125 (2014).
Google Scholar
Cao, X. et al. Differentiation and functional comparison of monocytes and macrophages from hiPSCs with peripheral blood derivatives. Stem Cell Rep. 12, 1282–1297 (2019).
Google Scholar
Ackermann, M. et al. Promoter and lineage independent anti-silencing activity of the A2 ubiquitous chromatin opening element for optimized human pluripotent stem cell-based gene therapy. Biomaterials 35, 1531–1542 (2014).
Google Scholar
Hong, D. et al. Human-induced pluripotent stem cell-derived macrophages and their immunological function in response to tuberculosis infection. Stem Cell Res. Ther. 9, 49 (2018).
Google Scholar
Lopez-Yrigoyen, M. et al. A human iPSC line capable of differentiating into functional macrophages expressing ZsGreen: a tool for the study and in vivo tracking of therapeutic cells. Philos. Trans. R. Soc. Lond. B Biol. Sci. https://doi.org/10.1098/rstb.2017.0219 (2018).
Neehus, A. L. et al. Impaired respiratory burst contributes to infections in PKCδ-deficient patients. J. Exp. Med https://doi.org/10.1084/jem.20210501 (2021).
Google Scholar
Kuo, H. H. et al. Negligible-cost and weekend-free chemically defined human ipsc culture. Stem Cell Rep. 14, 256–270 (2020).
Google Scholar
Ishida, T. et al. Live-cell imaging of macrophage phagocytosis of asbestos fibers under fluorescence microscopy. Genes Environ. 41, 14 (2019).
Google Scholar
Lewis, L. E., Bain, J. M., Okai, B., Gow, N. A. & Erwig, L. P. Live-cell video microscopy of fungal pathogen phagocytosis. J. Vis. Exp. https://doi.org/10.3791/50196 (2013).
