A high-throughput assay to globally identify PUF variants with new specificities To quantify the interaction between a PUF domain and its RNA target, we applied the yeast three-hybrid assay37 (Fig. 1B).…
NISDA assay mechanism As shown in Fig. 1, the key components of the assay include an Initiator and two probes (M1 and M2). The Initiator is a dsDNA composed of an…
HLA-typed peripheral blood mononuclear cells (PBMCs) Twenty-one HLA-typed donor PBMCs were selected using an in-house algorithm called “SampPick”17. The preselected donors were purchased from Cellular Technology Limited (CTL) ePBMC library.…
1. Work, T. H., A new virus disease in India. Summary of preliminary report on investigations of the Virus Research Center on an epidemic disease affecting forest villagers and wild…
An integrated annotation set of 3′-UTRs and poly(A) sites to evaluate APA Given the reliance of many scRNA-seq protocols on poly(A) priming, such methods enrich for both mRNA 3′-ends as…
1. Bomblies, K. & Weigel, D. Hybrid necrosis: Autoimmunity as a potential gene-flow barrier in plant species. Nat. Rev. Genet. 8, 382–393 (2007). CAS Article Google Scholar 2. Rieseberg, L.…
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