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The role of DNA-binding and ARNT dimerization on the nucleo-cytoplasmic translocation of the aryl hydrocarbon receptor

Plasmids The plasmid pEYFP-AHR-C1 used by29 encodes the human AHR (start at Ala 11). All pEYFP-AHR variants, which carry mutations at certain amino acids (AHRH39G, AHRR40D, AHRS36G, AHRS36G.H39G.R40D, AHRL118E.A121D.L122E, AHRL50D.A79D.F82D…

Removal behaviour of residual pollutants from biologically treated palm oil mill effluent by Pennisetum purpureum in constructed wetland

Removal of COD in constructed wetland The CW had a steady state of outflow values after 75 days of operation, and good plant growth was observed. The system started to release…

Development of a highly sensitive chemiluminescent enzyme immunoassay for fragmented cytokeratin 18 using new antibodies

Production of monoclonal antibodies Monoclonal antibodies were prepared in a manner similar to that reported previously25. Briefly, mice were immunized with either recombinant CK18 protein (Prospec, Israel) or the synthetic…

One-step construction of circularized nanodiscs using SpyCatcher-SpyTag

Chemicals and reagents 1,2-dioleoyl-sn-glycero-3-phosphocholine (PC), 1,2-dioleoyl-sn-glycero-3-phospho-l-serine (PS), 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine (PE), 1,2-dioleoyl-sn-glycero-3-phospho-(1′-rac-glycerol) (PG), 1,2-dipalmitoyl-sn-glycero-3-phospho-ethanolamine-N-(7-nitro-2-1,3-benzoxadiazol-4-yl) (NBD-PE) and N-(lissamine rhodamine B sulfonyl)-1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine (rhodamine-PE) were obtained from Avanti Polar Lipids. Nitrilotriacetic acid (Ni2+-NTA)-chelating Sepharose and…