Plasmids Mammalian expression vector pcDNA3.1+ was used to express proteins ZFP362 ZFP362-DNMT3A or ZFP362-DNMT3A derived fusion constructs. For creating pcDNA-ZFP362-DNMT3A, the truncated and fusion constructs were ordered as gBlocks® (Integrated…
Design rationale Given that there are many small-molecule hydroxyl-group-containing drugs, a method for synthesizing IPPEs from alcohols using a mild catalytic transisopropenylation strategy might be useful. However, such catalytic transisopropenylation…
Mutation design We selected IgG4 S228P as the development target (PDB: 5DK3) and analyzed the overlay crystal structures of CH1/CL and TCR Cα/Cβ (PDB: 3ARB) (Fig. 1A). It was found that…
Of the 1098 individuals analysed, 336 were typed with the MassARRAY EUROFORGEN NAME assay20, and 762 were typed with the custom AmpliSeq EUROFORGEN NAME panel21. Only the 102 loci that…
This illustration shows a glowing stream of material from a star, torn to shreds as it was being devoured by a supermassive black hole. The feeding black hole is surrounded…
By Bureau of Reclamation September 19, 2021 Canyon Ferry Reservoir, part of the Upper Missouri River Basin. Credit: Bureau of Reclamation The Bureau of Reclamation released the Missouri River Headwaters…
ESA’s Rosalind Franklin twin rover on Earth has drilled down and extracted samples 1.7 meters into the ground – much deeper than any other martian rover has ever attempted. Credit:…
Mistrust of government tops reasons for vaccine hesitancy. People’s trust in the government’s approval of a safe and effective vaccine against COVID-19 is the biggest driver of vaccine uptake, an…
This 3D view of a rock mound called “Faillefeu” was created from data collected by NASA’s Ingenuity Mars Helicopter during its 13th flight at Mars on September 4, 2021. Credit:…
New research shows how a racing heart may alter decision-making brain circuits. Mount Sinai study discovers that body-state monitoring neurons can hijack the decision-making process. Anxiety, addiction, and other psychiatric…
