Ravi Chandra, Health News, ET HealthWorld
Shahid Akhter, editor, ETHealthworld, spoke to Ravi Chandra, Co-Founder & CEO, MedPay, to know about growth of startups in healthcare and the challenges that they face. Startups in healthcare: Challenges…
Genetic analysis by targeted next-generation sequencing and novel variation identification of maple syrup urine disease in Chinese Han population
1. Miryounesi, M., Ghafouri-Fard, S., Goodarzi, H. & Fardaei, M. A New missense mutation in the BCKDHB gene causes the classic form of maple syrup urine disease (MSUD). J. Pediatr.…
Computational identification of multiple lysine PTM sites by analyzing the instance hardness and feature importance
Dataset construction Accurate identification of protein’s post-translational modifications often requires a rigorously processed benchmark dataset. As this study is related to the multi-class multi-label classification problem, a few steps have…
Evaluation of Angelica decursiva reference genes under various stimuli for RT-qPCR data normalization
Selection of candidate reference genes, evaluation of amplification specificity and PCR efficiency Here, 11 candidate reference genes were selected based on the transcriptome data of A. decursiva (unpublished). Table 1…
Comparative analysis of prophages carried by human and animal-associated Staphylococcus aureus strains spreading across the European regions
1. Gordon, R. J. & Lowy, F. D. Pathogenesis of methicillin-resistant Staphylococcus aureus infection. Clin. Infect. Dis. 46, S350 (2008). CAS PubMed Article PubMed Central Google Scholar 2. Deghorain, M.…
Reconstructing and counting genomic fragments through tagmentation-based haploid phasing
High coverage and specific mapping lead to the identification of archipelagos reflecting input DNA Integer counting of haploids in BIGseq is based on the deterministic property of tagmentation: a long…
The complete genome of 2,6-dichlorobenzamide (BAM) degrader Aminobacter sp. MSH1 suggests a polyploid chromosome, phylogenetic reassignment, and functions of plasmids
Genome statistics The MSH1 genome (based on substrain DK1) consists of a chromosome of 5,301,518 bp and seven plasmids. The genome contains two large plasmids pUSP1 of 367,423 bp and pUSP2 of…
Quantitative assessment of sensitizing potency using a dose–response adaptation of GARDskin
GARDskin dose–response: methodological description The conventional GARDskin protocol assays chemicals at single concentrations and performs hazard classifications into the categories: skin sensitizer and non-sensitizer. Although results from the assay are…
Generation of a more efficient prime editor 2 by addition of the Rad51 DNA-binding domain
Plasmid vectors Sequences encoding human RPA70-C and Rad51DBD were synthesized by GeneScript, after which they were amplified by PCR and cloned into the pCMV-PE2 (Addgene, no. 132775) plasmid to generate…
A new qualitative RT-PCR assay detecting SARS-CoV-2
Specimens for the initial assay evaluation The remaining specimens collected during routine clinical care (166 nasopharyngeal swabs), which would otherwise have been discarded, were used in the evaluation of our…
