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Structural and mechanical properties of biodegradable poly(lactic acid) and pectin composites: using bionucleating agent to improve crystallization behavior

Tensile properties The results of the tensile properties are shown in Fig. 2. When comparing the tensile strength of pure PLA before and after annealing, the pure PLA had a higher…

Engineering and validation of a dual luciferase reporter system for quantitative and systematic assessment of regulatory sequences in Chinese hamster ovary cells

Experimental workflow and vector designs One way to validate the all-in-one reporter system is through transient or stable transfection of mammalian cells. Within this framework, the schematics in Fig. 1 illustrate…

Wuschel2 enables highly efficient CRISPR/Cas-targeted genome editing during rapid de novo shoot regeneration in sorghum

Overcoming genotype dependence using integrating morphogenic genes The efficiency of Agrobacterium-mediated transformation technology through callus induction, proliferation, and then maturation (hereby referred to as “conventional transformation”, to distinguish it from…