Biotechnology

Cells HEK293T/17 is a human embryonic kidney cell line (ATCC, CRL-11268). The HEK-ACE-2 adherent cell line was obtained through BEI Resources (NR-52511). All adherent cells were cultured at 37 °C with…

Lipids 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC), 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC), 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC), L-α-phosphatidylcholine, hydrogenated (Soy) (HSPC), 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE) and 1,2-dioleoyl-sn-glycero-3-phospho-(1’-rac-glycerol) (sodium salt) (DOPG) were purchased from Lipoid (Germany). 1,2-dioleoyl-sn-glycero-3-[(N-(5-amino-1-carboxypentyl) iminodiacetic acid) succinyl] (nickel…

Design of a large and gated DNA channel Before designing our large and gated channel (LGC) (Fig. 1a–c) we examined whether previous nanoarchitecture principles could be applied. In the previous DNA…

Blackshaw, S. et al. Comprehensive analysis of photoreceptor gene expression and the identification of candidate retinal disease genes. Cell 107(5), 579–589 (2001). CAS  PubMed  Article  Google Scholar  Arshavsky, V. Y.…

SemiBin pipeline SemiBin is developed with Python. The input to SemiBin consists of a set of contigs and mapped short reads (in the form of BAM files) from one or…

Preparation and characterization of MgG rods Galvanic cells generally consist of two kinds of metals with different electrode potentials32,33. To engineer MgG, Mg rods were placed in a neutral solution…

The lumen of membrane nanopores defines their function in biology and technology. In nanopore sensing, channel width controls the entrance and passage of individual molecules and influences the electrical read-out…

This is an artists illustration of the OSIRIS-REx spacecraft firing thrusters near the surface of the asteroid Apophis. Credit: Heather Roper The extended mission, dubbed OSIRIS-APEX, will study the near-Earth…